FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33070412
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT6531821.9751250755509184Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG3017930.9125770794751514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1379880.41725515847821915Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT545990.16509924339618146Clontech Universal Primer Mix Long (96% over 26bp)
CCGAAGTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376480.11384194427332807No Hit
CCGAAGTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347430.10505765697748186No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)