FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30353922
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT8831672.9095647013918002Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG3184421.049096719692434Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1784030.5877428294109737Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTACAAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT466840.1537989061182934No Hit
AGTACAAGTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354230.11669991113504212No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG343050.11301669682092481Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTACAAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337510.11119156199979692No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAATTTGAAGTAGATAGA332830.10964975135667805Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTACAAGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305350.10059655552913394No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)