FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38963209
Sequences flagged as poor quality0
Sequence length150
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT12710723.262236434375824Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4859781.2472740630783261Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1383310.3550297923356364No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1368420.3512082385206003Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1356190.3480693800143618Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG996050.25563859486008966Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT874370.2244091342681759No Hit
AGAGTCAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT699290.17947443702596466No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTTGCAAAGCTGAAACT583850.14984648723363622Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT558690.14338911356094924No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTCCGACCATAAACGATG553250.14199292465874666Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT533920.13703183431323637No Hit
AGAGTCAATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT507410.13022797993871604No Hit
AGAGTCAATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT484510.12435064062613528No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCCGAGCCGCCTGGATA443870.11392028823909242Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAACTAACCTGTCTCACG431540.11075576449568106Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT425940.1093185112140019No Hit
AGAGTCAATTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420200.10784532660028079No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGACGGACCAGAGCGAAAG408620.10487329213566574Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTAGGAATAATGGAATAG398660.1023170345132507Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)