FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33049924
Sequences flagged as poor quality0
Sequence length150
%GC24

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3776941.142798391911582Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT1819330.550479329392709Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT1352540.40924148569902913Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1341100.40578005565156516Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGGCATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT632930.1915072482466223No Hit
CTGGCATATTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT602850.1824058657441996No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT569090.17219101623350178No Hit
CTGGCATAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT452970.13705629096151628No Hit
CTGGCATAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430510.1302605113403589No Hit
CTGGCATATTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT418450.12661148630780514No Hit
CTGGCATATAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT401070.12135277527415796No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG386740.11701691053813013Illumina Single End PCR Primer 1 (100% over 50bp)
CTGGCATAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385160.11653884589870767No Hit
CTGGCATATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381080.11530434986779395No Hit
CTGGCATATTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370710.11216667245588824No Hit
CTGGCATAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357830.10826953792692534No Hit
CTGGCATATTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357220.10808496866740148No Hit
CTGGCATATTATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354690.10731946009921232No Hit
CTGGCATATTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351830.10645410258734633No Hit
CTGGCATATATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348760.10552520483859508No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)