FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26746457
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG24583139.191172498099467Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA4553871.7026068162972017Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT4191871.5672617872340997Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3183241.1901538958973146Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG2461390.9202676825569831Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1420370.5310497760507121Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1132830.4235439482694848Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC973380.3639285756614418Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA603190.22552145878611138No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG355310.1328437631945046Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCGGCATCTCGTATGC338570.12658499030357553TruSeq Adapter, Index 11 (97% over 36bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAGACGTGTGCTCTTCCGA330800.12367993263556365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA295380.11043705713994195Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA285130.1066047738584591Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAGAAAAAAAAAAAAAAAAAAA273530.10226775082770775Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)