FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26746457
Sequences flagged as poor quality0
Sequence length150
%GC36

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3935131.4712715033621089Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT3757071.404698199840076Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG2076720.7764467645191286Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA909110.339899224783305Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC829790.31024296040406396Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC440950.16486295736291354Clontech Universal Primer Mix Long (96% over 26bp)
CAATGGAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT403000.15067416218903312No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG368880.13791733237789214Illumina Single End PCR Primer 1 (100% over 50bp)
CAATGGAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297870.11136802156637045No Hit
CAATGGAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285580.10677302044154859No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)