FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12735993
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT2823212.2167176128316024Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG2439461.915406203505294Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG792610.6223385958205222Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC493270.38730391890133736Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA361030.28347220354156916Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC347890.273154986815712Clontech Universal Primer Mix Long (96% over 26bp)
CATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT171010.1342730009352235Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC157030.12329623610816996Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC136510.10718441820751629Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATACCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129100.10136626174339135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)