FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11153545
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT2473612.2177791903829682Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG1426741.2791807447766608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC294030.2636202211942481Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACCTTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135620.12159362785553832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)