FastQCFastQC Report
Fri 6 Jan 2023
EGAF00002372772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30365130
Sequences flagged as poor quality0
Sequence length150
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT10691573.5210025446951816Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1124460.3703129214332361Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG704130.2318876948657885Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTTGCAAAGCTGAAACT699090.23022789627444376Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTCCGACCATAAACGATG567210.18679649980092297Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAACTAACCTGTCTCACG469260.1545391045584195Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGAACTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT464740.15305055502808648No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTTCCGGGAAACCAAAGT361190.11894893912853328Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGACGGACCAGAGCGAAAG340070.11199359265051721Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGAACTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325860.10731388273325358No Hit
CGAACTTATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321250.10579569394236087No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCCGAGCCGCCTGGATA320850.10566396389542873Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAA314430.10354969664216818Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)