FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26861945
Sequences flagged as poor quality0
Sequence length150
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT4293321.5982908162458078Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACCTTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2135490.7949871090868513No Hit
CACCTTACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770070.28667693273886163No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC768290.28601428526489797Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACCTTACATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT691770.25752788936169735No Hit
CACCTTACTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT675080.2513146386086339No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAACTAACCTGTCTCACG630870.23485641117945855Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACCTTACTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT618920.2304077385312195No Hit
CACCTTACTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT608800.22664032705003306No Hit
CACCTTACTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT604570.225065608614715No Hit
CACCTTACTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT601120.22378126379158322No Hit
CACCTTACTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT586470.21832745171654547No Hit
CACCTTACTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT574820.21399046122683968No Hit
CACCTTACTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT565520.21052831431231062No Hit
CACCTTACTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT561200.2089200912294326No Hit
CACCTTACTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT546350.20339182438203934No Hit
CACCTTACTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT504140.18767814467641863No Hit
CACCTTACTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500510.18632679055816695No Hit
CACCTTACTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT432800.16112012737722453No Hit
CACCTTACTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430290.16018571998416348No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTTGCAAAGCTGAAACT406980.15150801626613414Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACCTTACTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349960.13028096066759126No Hit
CACCTTACTTATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333460.12413844194826547No Hit
CACCTTACTTTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320010.11913135850736051No Hit
CACCTTACATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315070.11729232563018055No Hit
CACCTTACTTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312510.1163393045440306No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG305120.1135882007054962Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACCTTACTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299620.11154069446572092No Hit
CACCTTACAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298360.1110716293998815No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT298300.11104929296817487Clontech Universal Primer Mix Long (96% over 26bp)
CACCTTACATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279810.1041659492639122No Hit
CACCTTACTATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279500.10405054436676124No Hit
CACCTTACTTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271910.10122498575587137No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGATTGACAGATTGATAGC268670.10001881844371285Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)