FastQCFastQC Report
Fri 10 May 2024
EGAF00002372777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29034030
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG427951414.739648612335248Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA8113432.794455333965006Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT3567911.2288717756370713Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG1308710.45075037809081275Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA637810.21967670350964025No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCGTGGAGAACAAAAAGTTC582150.20050609577795436Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATAGGAAGCAGTGGTATCAACGCAG537590.18515858804306534Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC474060.16327736797130815Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC454520.15654733428325315Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC439590.1514050925758498Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA436450.15032360302720635Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA410140.14126182276452837Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT400200.13783825393856794Clontech Universal Primer Mix Long (96% over 26bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT393070.13538251493161646Clontech Universal Primer Mix Long (96% over 26bp)
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG392910.13532740718391487Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG391220.134745331598817Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC388470.13379816718519613Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT375450.12931377421598034Clontech Universal Primer Mix Long (96% over 26bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG371190.1278465304334259Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT370550.12762609944261957Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG361280.1244332943101595Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA355850.12256307512253724Clontech Universal Primer Mix Long (96% over 26bp)
TGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA348820.12014177845789922Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC348200.11992823593555563Clontech Universal Primer Mix Long (96% over 26bp)
TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT340640.11732439485665615Clontech Universal Primer Mix Long (96% over 26bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA338080.11644267089343091Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA335870.11568149512830289Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT335420.11552650458789221Clontech Universal Primer Mix Long (96% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCTCATCTCGTATGC334180.11509941954320499TruSeq Adapter, Index 13 (97% over 37bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC333400.11483076927315981Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA330270.11375272395874772Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC318420.10967130639459972Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT318020.10953353702534577Clontech Universal Primer Mix Long (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA316320.10894801720601653Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG312430.10760821009002194Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG303480.10452562045296503Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC302480.10418119702983017Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT297050.10231097784220791Clontech SMART CDS Primer II A (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)