FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26998849
Sequences flagged as poor quality0
Sequence length150
%GC35

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT6455682.391094524066563Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4837911.791894906334711Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1050730.38917584968159197Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA891520.330206669180601Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTTGCAAAGCTGAAACT378180.14007263791134206Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAACTAACCTGTCTCACG373550.13835774999149036Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACGCTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288110.10671195649858999No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGCCGTTCTTAGTTGGTG277450.10276364003517335Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAA277340.10272289755759588Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTCCGACCATAAACGATG274630.10171915106455094Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACGCTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270400.10015241760861732No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)