FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36250958
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG25654897.077024005820756Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCTCTGCTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG4223761.165144380460235Clontech SMART CDS Primer II A (95% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT3591680.9907820918829234Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT3286600.9066243159698013Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA3018970.8327973015223488Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT2865600.7904894540993924Clontech Universal Primer Mix Long (96% over 26bp)
ACCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT1862610.5138098695212413Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG1509970.41653244032888737Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1457450.40204454734685907Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG1423590.3927041045370443Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAAGCAGAAGACGGCATACGAGATGATCTGGTGACTGGAGTTCAGACGTG1339630.36954333730987193TruSeq Adapter, Index 7 (100% over 50bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1319450.3639765878738984Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1316530.3631710919198328Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA1218570.33614835778960656Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1211030.3340684127575332Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT1091070.30097687349393637Clontech Universal Primer Mix Long (96% over 26bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT982740.2710935253076622Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC969680.26749086189667043Clontech Universal Primer Mix Long (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC959650.26472403846541104Clontech Universal Primer Mix Long (96% over 25bp)
ACCTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT948960.2617751508801505Clontech Universal Primer Mix Long (95% over 23bp)
ACCTCCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940410.2594165925214997No Hit
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG908860.25071337425068874Clontech Universal Primer Mix Long (96% over 26bp)
TGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA902720.2490196259089208Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC853340.23539791693229184Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC838750.231373195709752Clontech Universal Primer Mix Long (96% over 26bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG838080.231188373007963Clontech Universal Primer Mix Long (96% over 26bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC829710.22887946850949428Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA819300.22600782026229488Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA817000.22537335427107885Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG809750.22337340712485446Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT766710.21150061744575135Clontech SMART CDS Primer II A (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG731160.20169398005978212Clontech Universal Primer Mix Long (96% over 26bp)
TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT724710.19991471673658942Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT701460.1935010931297319Clontech Universal Primer Mix Long (96% over 26bp)
ACCACCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG637230.17578294068807782Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT609810.16821900265366782Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC582370.16064954752368202Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCTCCAATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT525970.14509133799995025Clontech Universal Primer Mix Long (96% over 26bp)
ACCTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT488820.1348433329679177Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC487560.13449575594664284Clontech Universal Primer Mix Long (96% over 26bp)
ACCTCTGCTTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT480900.13265856311990432Clontech SMARTer II A Oligonucleotide (95% over 22bp)
ACCTCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT452760.1248960096447658Clontech Universal Primer Mix Long (96% over 26bp)
TGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC424130.1169982873280204Clontech SMART CDS Primer II A (96% over 26bp)
TCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT404010.11144808917877426Clontech SMART CDS Primer II A (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)