FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27049742
Sequences flagged as poor quality0
Sequence length150
%GC31

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT4075581.506698289395884Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1929750.7134079134654963Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1293960.47836315777059907Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCGAAGTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT776080.28690846663158565No Hit
CCGAAGTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT735630.27195453472347353No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC654360.24190988586878204Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCGAAGTATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT612060.22627202876833352No Hit
CCGAAGTATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT528780.1954843044344009No Hit
CCGAAGTATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT514550.19022362579280794No Hit
CCGAAGTATTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT461130.17047482375247794No Hit
CCGAAGTATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT455820.1685117736058259No Hit
CCGAAGTATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT436250.16127695413878626No Hit
CCGAAGTATTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT429660.15884070169689604No Hit
CCGAAGTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385960.1426852795860308No Hit
CCGAAGTAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344240.1272618422756121No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAACTAACCTGTCTCACG333000.12310653462055202Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCGAAGTATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320650.11854087185009012No Hit
CCGAAGTATTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301370.11141326227806535No Hit
CCGAAGTATTTTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295070.10908421973119004No Hit
CCGAAGTAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292340.10807496796087741No Hit
CCGAAGTATTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290860.10752782780700829No Hit
CCGAAGTAATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290020.10721728880075826No Hit
CCGAAGTATTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286970.10608973645663607No Hit
CCGAAGTATATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278890.1031026469679452No Hit
CCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTT276750.10231151188059391No Hit
CCGAAGTATTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276050.10205272937538555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)