FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30731899
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG334219110.875315580075283Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT10372033.375004583999186Clontech Universal Primer Mix Long (96% over 26bp)
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA5989421.94892609792841Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA4746481.5444798904226518Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCACCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4720811.5361270060141743Clontech Universal Primer Mix Long (96% over 26bp)
GCCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT3279641.0671777881347326Clontech Universal Primer Mix Long (96% over 26bp)
GCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG3055720.9943153854566553Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT2632260.8565237052223815Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1772630.5768045768990715Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA1660180.5402139321100854Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1621130.5275072653336521Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1618240.5265668743737574Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1576220.5128937850537645Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG1415130.46047593739651427Clontech Universal Primer Mix Long (96% over 26bp)
GCCACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG1402860.4564833432519091Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT1396240.45432922970363787Clontech Universal Primer Mix Long (96% over 26bp)
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1392140.4529951110408114Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1383670.45023901712028924Clontech SMART CDS Primer II A (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1335430.4345419721703498Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1296150.42176046459088Clontech Universal Primer Mix Long (96% over 26bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT1284140.4178524730931857Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC1268450.41274702874690555Clontech Universal Primer Mix Long (96% over 26bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG1210200.39379278189089456Clontech Universal Primer Mix Long (96% over 26bp)
TGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA1208000.3930769133401095Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG1207480.39290770804628766Clontech Universal Primer Mix Long (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC1190240.38729790176650003Clontech Universal Primer Mix Long (96% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1175350.38245277325686905Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG1125240.36614724003876237Clontech Universal Primer Mix Long (96% over 26bp)
TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT1124640.3659520031612755Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC1111370.3616340142208589Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC1097090.35698737653667284Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA1095770.3565578554062019Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT1095230.35638214221646375Clontech Universal Primer Mix Long (96% over 26bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC1091500.35516841962808743Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT1036980.33742789536045265Clontech SMART CDS Primer II A (96% over 26bp)
ACCACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT869830.28303815524058573Clontech Universal Primer Mix Long (96% over 26bp)
TGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC859950.27982325465796953Clontech SMART CDS Primer II A (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC850110.2766213698671859Clontech Universal Primer Mix Long (96% over 26bp)
TCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT827840.26937482776446714Clontech SMART CDS Primer II A (96% over 25bp)
ACCACTGCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG755920.24597243404971492Clontech Universal Primer Mix Long (96% over 26bp)
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT628940.20465380287758983Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC620900.2020376287192666Clontech SMART CDS Primer II A (96% over 26bp)
GCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGAT612580.19933034401811617Clontech SMART CDS Primer II A (96% over 26bp)
GCCACATATACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG608810.19810360563790735Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT587880.19129309256157584Clontech Universal Primer Mix Long (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA543890.1769789754938346Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC494210.1608133620379268Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA431170.14030047410997934Clontech SMARTer II A Oligonucleotide (100% over 24bp)
ACCACCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT426390.1387450869860011Clontech SMART CDS Primer II A (96% over 26bp)
ACCACTGCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC397680.12940300239825728Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAG376880.1226347906453812Clontech SMART CDS Primer II A (96% over 26bp)
CAAGCAGAAGACGGCATACGAGATTCTGAGGTGACTGGAGTTCAGACGTG371950.12103059430203125RNA PCR Primer, Index 40 (97% over 43bp)
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG363930.11842092803962423Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCT329210.1071232207290542Clontech SMART CDS Primer II A (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA316690.1030492778854961Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT311970.10151341444926655Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA307640.1001044549834034Clontech SMART CDS Primer II A (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)