FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30158901
Sequences flagged as poor quality0
Sequence length150
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT7262972.4082343053548274Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6874522.279433192873971Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC2222050.7367808263305086Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1316220.43642836985339756Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACAAGCTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT679180.22520051377203698No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGCCGTTCTTAGTTGGTG501470.16627595282732616Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAACTAACCTGTCTCACG417400.13840026863047827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTTGCAAAGCTGAAACT385410.12779311819087838Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGATTGACAGATTGATAGC376660.12489181883650204Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA340870.11302467553443012Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC336740.11165526223916447Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACGGACAGGATTGACAGATT324050.10744754923264611Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC319350.10588913700800967Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG318190.10550450760788664Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT310620.10299446919501476Clontech Universal Primer Mix Long (96% over 26bp)
ACAAGCTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310260.102875101450149No Hit
ACAAGCTATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304620.10100500678058527No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA303630.10067674548220441Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG303510.10063695623391582Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)