FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33274817
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT9393192.8229125948311Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6188971.859956134394368Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG2553410.7673701105553788Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1558650.46841730188929365Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC985340.296121838927018Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT977370.29372663416901734Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA933460.2805304684320277Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC924750.27791287326989655Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGTAGCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG579520.17416173919153335Clontech Universal Primer Mix Long (96% over 26bp)
CTGTAGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT577400.17352462073645664No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCCGAGCCGCCTGGATA449740.13515927074820577Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC347270.10436421032758798Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT336210.10104037536855576Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)