FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32149281
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT11769783.660977674741777Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6595632.0515637659206125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG1938580.6029932675632778Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1465250.45576446950710964Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1152910.358611441419172Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT728310.2265400585474991No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAA490400.152538403580472Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT441860.1374400876958959No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC395160.12291410187369355Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCCGAGCCGCCTGGATA376690.11716902782367045Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT356590.11091694399013154No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTCCGACCATAAACGATG349580.10873649087206647Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAAGCAGAAGACGGCATACGAGATGATCTGGTGACTGGAGTTCAGACGTG322930.1004470364360559TruSeq Adapter, Index 7 (100% over 50bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTTGCAAAGCTGAAACT322810.10040971056242283Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)