FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37479937
Sequences flagged as poor quality0
Sequence length150
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT10088902.691813489441031Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4228111.1280995482996676Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC2285250.6097262116529172Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAACTAACCTGTCTCACG1060150.282857999467822Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTCACTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1044330.27863707455004527No Hit
AGTCACTATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT977990.2609369380743623No Hit
AGTCACTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT948170.25298068137094254No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTTGCAAAGCTGAAACT880100.23481896461031937Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTCACTATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT689150.1838717071482804No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA654750.17469346333212887Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTCACTATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT641150.1710648553117899No Hit
AGTCACTATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT633270.16896239713529934No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTCCGACCATAAACGATG585390.15618756242840004Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTTCCGGGAAACCAAAGT529060.14115818817945186Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTCACTAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT489920.13071526774444686No Hit
AGTCACTATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT459450.12258558492240795No Hit
AGTCACTATTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440800.11760958936510486No Hit
AGTCACTATTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT437410.1167051054541527No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAA431140.11503221043301112Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTCACTATATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT424050.11314053169299618No Hit
AGTCACTATAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421090.11235077582974592No Hit
AGTCACTATATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416120.11102473304584265No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGACGGACCAGAGCGAAAG383070.10220668193759237Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTCACTATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379240.10118480188480573No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)