FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28216038
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG322567511.43206214848449Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA4541891.6096838259148927Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3205631.1361020990969746Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1803420.6391471403603864Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT1689330.5987126895703784Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1670830.5921561347486135Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA1660050.5883356125335527Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT1608190.5699559945304865Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1601210.5674822241166531Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG1576210.558622014898052Clontech Universal Primer Mix Long (96% over 26bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT1567470.555524485755229Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG1567180.5554217073282932Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1537540.5449170432787197Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1511020.5355181333396276Clontech Universal Primer Mix Long (96% over 26bp)
TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT1499170.5313183941700107Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA1472000.5216891187912349Clontech Universal Primer Mix Long (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC1462700.5183931209619154Clontech Universal Primer Mix Long (96% over 25bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG1461550.5179855513378597Clontech Universal Primer Mix Long (96% over 26bp)
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1444650.5119960499060853Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1442250.5111454698210997Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC1439780.5102700811503018Clontech Universal Primer Mix Long (96% over 26bp)
TGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA1388940.49225195968335456Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1355730.4804820577573648Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT1326710.47019712689641263Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC1303000.46179410447349134Clontech Universal Primer Mix Long (96% over 26bp)
CATCAAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1301580.46129084458987474No Hit
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC1298490.46019572273045567Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT1284620.45528007865597575Clontech SMART CDS Primer II A (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG1263360.44774535673647736Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC1238920.43908361620437286Clontech Universal Primer Mix Long (96% over 26bp)
TCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT1198410.4247265331865516Clontech SMART CDS Primer II A (96% over 25bp)
TGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC1043110.3696869135206013Clontech SMART CDS Primer II A (96% over 26bp)
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT1034380.3665929284614658Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC913580.32378039751718507Clontech SMART CDS Primer II A (96% over 26bp)
CATCAAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC851110.3016405067217446Clontech SMART CDS Primer II A (95% over 24bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA685430.24292212818823108Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG601900.21331839714704096Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT577770.20476652320924718Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA570730.2022714882932891Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG559460.1982773059775437Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT543320.1925571549060148Clontech Universal Primer Mix Long (100% over 23bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA518720.18383870903491129Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG501500.17773579692513883Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC486720.1724976412351018Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC475040.16835815148817135Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT460930.1633574494051929Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG457420.1621134760309013Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA457210.16203905027346505Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG442290.1567512774118039Illumina Single End PCR Primer 1 (100% over 50bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG433700.15370690952429253Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC432790.15338439790873545Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA432330.15322137005911318Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATAGGAAGCAGTGGTATCAACGCAG428860.15199157301957136Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG428530.1518746182578858Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG422780.14983677013760754Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG419890.14881252995193728Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC416200.14750476307127175Clontech SMART CDS Primer II A (96% over 26bp)
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA401420.14226660738123476Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACA400370.1418944785940535Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA400270.1418590377571791Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG390330.13833621857186326Clontech Universal Primer Mix Long (96% over 26bp)
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT377710.1338635849583134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC367570.1302698840992488Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT350520.12422722141216282Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA348500.12351131650729986Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC342460.12137068996008582Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG335480.11889691954625238Clontech Universal Primer Mix Long (96% over 26bp)
CATCAAGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291350.10325687823357767No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA282580.10014871683969237Clontech Universal Primer Mix Long (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)