FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20529576
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG19251369.37737827610273Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCTCTGCTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG4228122.0595262172000046Clontech SMART CDS Primer II A (95% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA3102081.5110297455729238Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT2822951.375064930712646Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG1526840.7437270014733866Clontech Universal Primer Mix Long (96% over 26bp)
ACCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1430760.6969262297477552Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1324420.6451277902670761Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA1289700.6282156046476557Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1229480.5988823149586723Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1224720.5965637088656873Clontech Universal Primer Mix Long (96% over 26bp)
ACCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT1095360.5335521785739754Clontech Universal Primer Mix Long (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC1042870.5079841882754909Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1033120.5032349426018345Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT1019720.4967077741888094Clontech Universal Primer Mix Long (96% over 26bp)
ACCTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT979290.4770142354620475Clontech Universal Primer Mix Long (95% over 23bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT978550.47665377989297003Clontech Universal Primer Mix Long (96% over 26bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC948490.4620114901544971Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC853780.4158780483337795Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC849840.41395886597950193Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT815190.39708077750850773Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC800150.3897547616180675Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA786110.3829158478480023Clontech Universal Primer Mix Long (96% over 26bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG778610.3792625819451897Clontech Universal Primer Mix Long (96% over 26bp)
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG756680.36858043244536565Clontech Universal Primer Mix Long (96% over 26bp)
TGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA710350.34601299120839124Clontech Universal Primer Mix Long (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA654610.31886191901868793Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG634060.3088519704449814Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT632910.3082918030065502Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG608620.29646009250264105Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA580060.28254845594473066Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT541500.2637657981830701Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT528110.2572435007912487Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT507150.2470338403481884Clontech Universal Primer Mix Long (96% over 26bp)
ACCTCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT481090.2343399590912155Clontech Universal Primer Mix Long (96% over 26bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA451640.21999480164617136Clontech SMARTer II A Oligonucleotide (100% over 24bp)
ACCTCTGCTTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT433980.21139257820034862Clontech SMARTer II A Oligonucleotide (95% over 22bp)
ACCACCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG424540.2067943341840085Clontech Universal Primer Mix Long (96% over 26bp)
ACCTCCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT399270.19448526360213186No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC381700.1859268793471429Clontech Universal Primer Mix Long (96% over 26bp)
TGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC372170.18128479613996898Clontech SMART CDS Primer II A (96% over 26bp)
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG362970.17680345663251884Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT344790.1679479400841011Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT329820.16065602134208715Clontech SMART CDS Primer II A (96% over 25bp)
ACCTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT328150.15984256080106088Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT289330.14093325648810281Clontech Universal Primer Mix Long (100% over 23bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA273550.1332467850285851Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG269420.1312350532714363Clontech Universal Primer Mix Long (96% over 26bp)
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA269100.1310791805929163Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT256520.1249514359185986Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC250150.12184859541180977Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCTCCAATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT245780.11971995914577095Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA244410.11905262924085719Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG234800.11437157786405332Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG232740.11336814749608078Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC225390.10978794691132443Clontech SMART CDS Primer II A (96% over 26bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG225310.10974897874169443Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC224230.1092229084516894Clontech Universal Primer Mix Long (96% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA221140.10771776289973062Clontech Universal Primer Mix Long (96% over 26bp)
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT218690.10652436270481183Clontech Universal Primer Mix Long (96% over 26bp)
ACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA209860.10222325098190047Clontech Universal Primer Mix Long (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)