FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002372856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28298139
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG316734211.192757233965104Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT294474610.406147202824892Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA4009201.4167716117303686Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2676140.9456946974498923Clontech Universal Primer Mix Long (96% over 26bp)
CATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTA2282350.8065371365940355Clontech SMART CDS Primer II A (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC2057010.7269064584070352Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1900540.6716130696792464Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA1796460.6348332658907357Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1750120.6184576307297098Clontech Universal Primer Mix Long (96% over 26bp)
CATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTAGATCGG1652650.5840136695914879Clontech SMART CDS Primer II A (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT1642900.5805682133372798Clontech Universal Primer Mix Long (96% over 26bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT1541800.5448414823321067Clontech Universal Primer Mix Long (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC1476720.5218435035604285Clontech Universal Primer Mix Long (96% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1376070.4862757936131419Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC1335170.4718225463518997Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC1331560.4705468440875211Clontech Universal Primer Mix Long (96% over 26bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG1308380.46235549270572174Clontech Universal Primer Mix Long (96% over 26bp)
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1295800.45790997068747175Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA1280720.4525809983476298Clontech Universal Primer Mix Long (96% over 26bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC1266710.4476301427454293Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1249520.44155553833416394Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG1233110.4357565704232353Clontech Universal Primer Mix Long (96% over 26bp)
TGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA1211700.4281907018691229Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT1185430.4189074058898361Clontech SMART CDS Primer II A (96% over 26bp)
CATACCACTGCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA1165080.41171612027207866Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1111800.3928880270183138Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT1084440.383219546698813Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG1083520.38289443698046716Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG1036680.3663421117551228Clontech Universal Primer Mix Long (96% over 26bp)
CATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA1015170.35874090518814683Clontech Universal Primer Mix Long (96% over 26bp)
TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT987430.348938140419764Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT842710.29779696820345675Clontech Universal Primer Mix Long (96% over 26bp)
CATACCACTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC833840.294662486462449Clontech Universal Primer Mix Long (96% over 26bp)
CATACCACCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT733690.2592714665794807Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC689080.2435071790409963Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT660080.2332591553105312Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC597700.21121530288617216Clontech SMART CDS Primer II A (96% over 26bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA596140.21066402988549884Clontech SMARTer II A Oligonucleotide (100% over 24bp)
TCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT557160.1968892724712392Clontech SMART CDS Primer II A (96% over 25bp)
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG501830.17733674995376902Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA480840.16991930105368414Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CATACCACTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATG477660.16879555224461934Clontech Universal Primer Mix Long (96% over 26bp)
CATACCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT467700.16527588616339753No Hit
CATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC459140.16225095226226713Clontech SMART CDS Primer II A (96% over 26bp)
CATACCACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG453620.16030029395219242Clontech Universal Primer Mix Long (96% over 26bp)
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT444800.15718348121761647Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA425730.15044452216451407Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATACCACTGCTTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT407140.14387518557315732Clontech Universal Primer Mix Long (96% over 26bp)
CATACCACTGCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT375540.13270837350823672Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG375420.1326659678928003Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT371740.13136552901941714Clontech Universal Primer Mix Long (100% over 23bp)
CATACCACTGCTTGCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG367430.12984246066499286Clontech Universal Primer Mix Long (96% over 26bp)
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT359430.12701541963589902Clontech Universal Primer Mix Long (96% over 26bp)
CATACCACTGCTTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG352170.12444987990199638Clontech Universal Primer Mix Long (96% over 26bp)
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA351640.1242625884338189Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA349780.12360530139455461Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC349640.12355582817654547Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT347990.12297275096429486Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG340510.12032946760209215Clontech Universal Primer Mix Long (96% over 26bp)
CATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT332530.11750949417557105Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA327200.1156259780899373Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG326390.11533974018574154Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC322910.11410997733808573Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC314550.11115571946268268Clontech Universal Primer Mix Long (96% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC310780.10982347637772223Clontech SMART CDS Primer II A (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)