FastQCFastQC Report
Wed 22 Feb 2023
EGAF00002372860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17114368
Sequences flagged as poor quality0
Sequence length150
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT6483253.7881913021853917Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1912301.1173652453891374Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC948630.5542886538375241Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830840.4854634421791094No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAACTAACCTGTCTCACG548940.32074804047686717Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT488340.285339195698024No Hit
AGAGTCAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416430.24332186850253543No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTTGCAAAGCTGAAACT367250.2145857796209594Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA354180.20694892151436736Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGCCGTTCTTAGTTGGTG351600.20544141624160472Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAA342550.20015346169954978Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327580.19140642529131077No Hit
AGAGTCAATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316210.18476288461250803No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGATTGACAGATTGATAGC303880.17755841173918896Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300730.17571785297593226No Hit
AGAGTCAATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT297550.17385976508159695No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTCCGACCATAAACGATG270310.15794331406219617Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAATTTGAAGTAGATAGA255200.1491144750422569Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTTCCGGGAAACCAAAGT247840.14481399488429839Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247830.1448081518406055No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACGGACAGGATTGACAGATT236060.13793088941408763Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224270.131041940900184No Hit
AGAGTCAAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216040.12623311594094505No Hit
AGAGTCAATATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188570.11018227491660809No Hit
AGAGTCAATAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181720.10617978998698636No Hit
AGAGTCAATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178620.10436844644219406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)