FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22739975
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG9147714.022744088329032Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3224861.4181457983133228Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT1958950.8614565319442963Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG1077740.4739407145346466Clontech Universal Primer Mix Long (96% over 26bp)
CCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT1033010.4542705082129598Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATAGGAAGCAGTGGTATCAACGCAG553790.24353149025009924Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA533370.23455170904981207Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTCCTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT525920.23127554010063775No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC505280.22219901297165015Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC477770.2101013743418803Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA472930.20797296391047043Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG470150.20675044717507388Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTCCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT458680.20170646625600952Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA450300.19802132588096513Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT445090.19573020638764993Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG436420.19191753728840952Clontech Universal Primer Mix Long (96% over 26bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA379830.16703184590132575Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA374030.16448127141740482Clontech Universal Primer Mix Long (96% over 26bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT373650.16431416481328584Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC368310.16196587727119313Clontech Universal Primer Mix Long (96% over 26bp)
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG366430.16113913933502563Clontech Universal Primer Mix Long (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC362370.15935373719628101Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA349960.1538963873091329Clontech Universal Primer Mix Long (96% over 26bp)
TGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA349090.15351380113654478Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG348510.1532587436881527Clontech Universal Primer Mix Long (96% over 26bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC342740.15072136183087273Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC332100.1460423769155419Clontech Universal Primer Mix Long (96% over 26bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG328980.144670343744881Clontech Universal Primer Mix Long (96% over 26bp)
TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT325860.14329831057422007Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT325510.14314439659674208Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT325060.14294650719712754Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG322940.1420142282478323Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT311920.13716813672838252Clontech Universal Primer Mix Long (96% over 26bp)
CCTCCTGAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310860.13670199725373489No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC305700.13443286547148797Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT294730.12960876166310648Clontech SMART CDS Primer II A (96% over 26bp)
CCTCCTGATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292810.1287644335580844No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC276950.12178993160722473Clontech Universal Primer Mix Long (96% over 26bp)
TCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT255290.11226485517244413Clontech SMART CDS Primer II A (96% over 25bp)
TGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC250340.11008807177668402Clontech SMART CDS Primer II A (96% over 26bp)
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT244130.10735719806200315Clontech Universal Primer Mix Long (96% over 26bp)
CCTCCTGATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237080.10425693080137512No Hit
CCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT234810.10325868871887503Clontech Universal Primer Mix Long (96% over 26bp)
CCTCCTGATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT233090.10250231145812606No Hit
CCTCCTGATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229460.10090600363456864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)