FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33023796
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC27721188.394304519080725Clontech Universal Primer Mix Long (96% over 26bp)
ATCATTCCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC25930237.851983460653646Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG23218227.030754429321208Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA3795891.1494408456253788Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCG3043790.9216959794688654Clontech SMART CDS Primer II A (96% over 26bp)
ATCATTCCTATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCG2992490.9061617265319831Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG2226380.6741744649827658Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT2205940.6679849887638599Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1801340.5454672745677087Clontech Universal Primer Mix Long (96% over 26bp)
ATCATTCCCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCG1641090.49694165988670713Clontech SMART CDS Primer II A (96% over 26bp)
ATCATTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1581300.4788365335105631Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1224630.37083259598623974Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1222000.37003620056277Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA1205230.3649580441933447Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1154130.34948435364607994Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT1019840.3088197371374266Clontech Universal Primer Mix Long (96% over 26bp)
ATCATTCCTATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCG943610.28573638233472615Clontech SMART CDS Primer II A (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC943180.2856061731970486Clontech Universal Primer Mix Long (96% over 25bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT925210.2801646424899185Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC920580.27876262317027395Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG880080.2664987392727353Clontech Universal Primer Mix Long (96% over 26bp)
TGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG864830.2618808570644029Clontech Universal Primer Mix Long (96% over 26bp)
ATCATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG853800.25854084127699917Clontech Universal Primer Mix Long (96% over 26bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC826880.25038914363448705Clontech Universal Primer Mix Long (96% over 26bp)
TGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA825460.24995915066820304Clontech Universal Primer Mix Long (96% over 26bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG820110.24833910674593557Clontech Universal Primer Mix Long (96% over 26bp)
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT814120.24652526317689222Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC794820.24068099257880587Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC791220.23959086956569137Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA786530.23817068152916157Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT784140.23744696097323276Clontech SMART CDS Primer II A (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG762440.23087594169973677Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA755830.22887435472287923Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG691210.20930664663747317Clontech Universal Primer Mix Long (96% over 26bp)
ATCATTCCTATGTACTCTGCGTTGATACCACTGCTCCCATGTACTCTGCG690620.20912798758810164Clontech SMART CDS Primer II A (96% over 25bp)
TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT668090.2023056343976931Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT667120.20201190680804834Clontech Universal Primer Mix Long (96% over 26bp)
ATCATTCCTATGTACTCTGCGTTGATACCACTGCTTCCTATGTACTCTGC631510.191228773336657Clontech SMART CDS Primer II A (96% over 26bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA611620.18520584368919915Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT608030.18411874879556547Clontech Universal Primer Mix Long (96% over 26bp)
ATCATTCCTATGTACTCTGCGTTGATACCACTGCCCCATGTACTCTGCGT576880.1746861566126438Clontech SMART CDS Primer II A (95% over 24bp)
ATCATTCCTATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGC537120.16264635355668985Clontech Universal Primer Mix Long (96% over 26bp)
ATCATTCCCATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGC496850.15045211640721132Clontech Universal Primer Mix Long (96% over 26bp)
ATCATTCCTATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTG461330.13969623601114783Clontech Universal Primer Mix Long (96% over 27bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC456910.13835780720060165Clontech Universal Primer Mix Long (96% over 26bp)
ATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCCTATGTACTCTGC449310.13605643639513762Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG448770.13589291794317043Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC435990.1320229812466138Clontech SMART CDS Primer II A (96% over 26bp)
ATCATTCCTTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG407830.12349579678847337Clontech Universal Primer Mix Long (96% over 26bp)
TCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT401290.12151540664798197Clontech SMART CDS Primer II A (96% over 25bp)
ATCATTCCTATGTACTCTGCGTTGATACCCATGTACTCTGCGTTGATACC362680.10982383733232849No Hit
ATCATTCCTATGTACTCTGCGTTGATACCACTCCCATGTACTCTGCGTTG362100.1096482064024378Clontech SMARTer II A Oligonucleotide (95% over 23bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA353950.1071802890255257Clontech SMARTer II A Oligonucleotide (100% over 24bp)
ATCATTCCTTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG350460.10612347532670079Clontech Universal Primer Mix Long (96% over 26bp)
ATCATTCCCATGTACTCTGCGTTGATACCACTGCTCCCATGTACTCTGCG349520.10583883209549864Clontech SMART CDS Primer II A (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)