FastQCFastQC Report
Wed 22 Feb 2023
EGAF00002372928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22914797
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT6474552.8254887005981333Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2833161.236388871348064Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1165280.5085273066132769Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT868280.37891673227565575Clontech Universal Primer Mix Long (96% over 26bp)
GCTAACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT802790.35033694603535No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC643990.28103674669254103Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGCCGTTCTTAGTTGGTG580310.25324684307698647Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAACTAACCTGTCTCACG516570.22543075550702021Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTAACGAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT492450.21490480583354066No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA434420.1895805579250822Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGATTGACAGATTGATAGC427740.18666541100058623Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTTGCAAAGCTGAAACT426420.18608936400353013Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTAACGATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT362560.1582209085247406No Hit
GCTAACGATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347480.1516400079826149No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAA337490.14728037957307671Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACGGACAGGATTGACAGATT336780.14697053611253896Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTAACGATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328320.14327859854049765No Hit
GCTAACGATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT298570.13029572114472585No Hit
GCTAACGATTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296900.12956693441360181No Hit
GCTAACGATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291740.12731511433420073No Hit
GCTAACGATTTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257980.11258227598525093No Hit
GCTAACGATTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253170.11048319563991774No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTTCCGGGAAACCAAAGT246280.10747640487498099Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTAACGAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231050.10083004444682621No Hit
GCTAACGATTTTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230550.1006118448267292No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)