FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28954774
Sequences flagged as poor quality0
Sequence length150
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT8174732.8232753603948004Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3676151.2696179220739212Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACTCACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1013210.3499284781155605Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG993700.34319038373430233Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC877780.3031555349041923Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT685340.23669326515896827Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA513650.17739734387151493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACTCACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325090.11227509494634633No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCCGAGCCGCCTGGATA324080.11192627509370302Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACTCACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC319650.11039630286874282Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)