FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372936

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372936
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33070109
Sequences flagged as poor quality0
Sequence length150
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT8712132.6344424809727722Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC2784410.841971824163023Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1193960.36103902772137825Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTACAAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT954970.28877134937777194No Hit
AGTACAAGTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT732300.22143864116081383No Hit
AGTACAAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT726630.21972410190725408No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGCCGTTCTTAGTTGGTG657890.19893795935175176Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTACAAGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT614950.18595342398175949No Hit
AGTACAAGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT542010.16389725234954622No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG452930.1369605404082581Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTACAAGAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT446570.13503735352066726No Hit
AGTACAAGTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT432820.130879520233816No Hit
AGTACAAGATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT431310.13042291454195085No Hit
AGTACAAGTATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT426940.12910147952642068No Hit
AGTACAAGTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT426050.12883235431730813No Hit
AGTACAAGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT422750.12783447432846382No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAA399810.12089769646661884Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTACAAGTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT389000.11762888353346523No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCCGAGCCGCCTGGATA366200.11073443997417728Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTACAAGTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT362790.10970329731903818No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGATTGACAGATTGATAGC360910.10913480811327232Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTAAAAAGCTCGTAGTTG349360.10564222815231725Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTACAAGTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349030.10554244015343282No Hit
AGTACAAGATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333680.10090078626592977No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)