FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42898652
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT18203774.243436367184684Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACAGAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1874390.436934475237124No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1794160.4182322558760121Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG1166050.27181506775550895Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTTGCAAAGCTGAAACT908230.2117152772073118Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACAGAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT881070.20538407593786398No Hit
ACACAGAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT856000.19954006946418737No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG778570.18149055126487423Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTCCGACCATAAACGATG769230.17931332667515987Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCCGAGCCGCCTGGATA630350.14693934904994216Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAACTAACCTGTCTCACG615100.14338445879371686Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGACGGACCAGAGCGAAAG595380.13878757775419143Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACAGAATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT578010.13473849947546138No Hit
ACACAGAATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT569200.13268482189137318No Hit
ACACAGAATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT562300.13107637974265485No Hit
ACACAGAATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540290.12594568239580115No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTAGGAATAATGGAATAG514680.11997579784092051Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTTCCGGGAAACCAAAGT512920.11956552853921844Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAA508530.11854218636054112Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA484920.113038516921231Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACAGAATTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT469070.10934376213033455No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGCGCCGCTAGAGGTGAA467820.10905237768310296Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACAGAATTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT432400.10079570798634885No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)