FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28318676
Sequences flagged as poor quality0
Sequence length150
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT9366643.3075840127553984Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAGCCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1192350.42104722692543955No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1084200.38285688215084634Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAGCCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT673890.2379666337508152No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG652120.23027912745638252Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAGCCATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT565060.19953616475572514No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTTGCAAAGCTGAAACT560490.1979223887444455Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAGCCATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT556640.19656286190780953No Hit
CTGAGCCATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT542080.1914213785983497No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG537130.18967341552267486Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTCCGACCATAAACGATG481380.16998676068047813Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAGCCATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT466360.16468284039832937No Hit
CTGAGCCATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT443010.15643739841509538No Hit
CTGAGCCATTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT429650.15171966373004162No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAACTAACCTGTCTCACG367030.1296070480131204Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAGCCATTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342810.12105438827719206No Hit
CTGAGCCATTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318760.11256175959638791No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCCGAGCCGCCTGGATA288470.10186563806867242Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)