FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002372996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002372996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31668662
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT9720153.069327652680748Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG1337140.422228132025281Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1122930.3545871309624638Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1048320.3310275628316725Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAGCCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT980290.3095457585167318No Hit
CTGAGCCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT529110.16707684082137728No Hit
CTGAGCCATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT482690.15241881706274804No Hit
CTGAGCCATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT472960.14934637907973503No Hit
CTGACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT460940.14555082876567377Clontech Universal Primer Mix Long (96% over 26bp)
CTGAGCCATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT455000.14367515747902454No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTTGCAAAGCTGAAACT422890.1335357963655048Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAGCCATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT412530.13026442354905932No Hit
CTGAGCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT390570.12333012364084091Clontech Universal Primer Mix Long (96% over 26bp)
CTGAGCCATTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354860.11205399205056404No Hit
CTGAGCCATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354020.11178874560598741No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)