FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36689357
Sequences flagged as poor quality0
Sequence length150
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT7330991.998124415208476Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1450140.39524813694609034Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1281790.34936289562120154Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT556970.15180696679966346No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT536970.14635579467909454Clontech Universal Primer Mix Long (96% over 26bp)
CCAGTTCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT461030.12565769413729438No Hit
CCAGTTCATTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT453760.12367619307146757No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)