FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52949465
Sequences flagged as poor quality0
Sequence length150
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG9016301.7028122947040165Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT3859750.7289497637039393Clontech Universal Primer Mix Long (96% over 26bp)
GATAGACATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT955110.1803814259501961No Hit
GATAGACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT621800.11743272571309266Clontech Universal Primer Mix Long (96% over 26bp)
GATAGACAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT582970.11009931828395245No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA570880.10781600909470945Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT563490.10642033871352619No Hit
GATAGACATTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT554650.10475082231709047No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)