FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48023634
Sequences flagged as poor quality0
Sequence length150
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1846890.38457939272150876Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA665030.13847973270827443Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGGACACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT619720.12904479490244325No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT617800.12864499175551772No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)