FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34590669
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT12549443.627984182670766Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1682630.48644043282308297Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACAGAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1641590.47457596151147No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG1468400.4245075456620975Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACAGAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750360.2169255529576488No Hit
ACACAGAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT705510.20395962853450447No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTTGCAAAGCTGAAACT602210.17409608354206738Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACAGAATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT493170.14257313149971168No Hit
ACACAGAATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT491460.14207877852839446No Hit
ACACAGAATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT479580.13864432630661178No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGCCGTTCTTAGTTGGTG466970.13499883451227845Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACAGAATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT463220.13391472711903896No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAA429970.12430230823231549Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACAGAATTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT404580.11696217844182198No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTCCGACCATAAACGATG398340.11515822373947147Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACAGAATTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT386760.11181050010914792No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTTCCGGGAAACCAAAGT347470.10045194558104673Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)