FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28364257
Sequences flagged as poor quality0
Sequence length150
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT2352150.8292655083473541Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTCCTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1726520.6086956552396208No Hit
CCTCCTGAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1002660.35349418812556943No Hit
CCTCCTGATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870130.306769890006285No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT817580.28824305180988874Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG746220.2630846279527082Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTCCTGATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT684110.2411873506857592No Hit
CCTCCTGATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT664790.23437596126702703No Hit
CCTCCTGATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT661380.23317374398349303No Hit
CCTCCTGATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT556390.19615884879339518No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG547380.19298231573631558Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTCCTGATTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT464440.16374128890455336No Hit
CCTCCTGATTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT449700.15854460774347093No Hit
CCTCCTGAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT422740.1490396875194016No Hit
CCTCCTGATTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT373940.1318349357785046No Hit
CCTCCTGATAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT358230.12629627492093304No Hit
CCTCCTGATATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339220.11959417798252216No Hit
CCTCCTGAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335280.1182051058132776No Hit
CCTCCTGATTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326640.1151590186198073No Hit
CCTCCTGAATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322900.11384045772818939No Hit
CCTCCTGATTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321270.11326579081553237No Hit
CCTCCTGAATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319090.11249721788940215No Hit
CCTCCTGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307500.10841108935093911No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC304270.10727233221726906Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTCCTGATATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294340.10377144728310704No Hit
CCTCCTGATTATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293730.10355638788634583No Hit
CCTCCTGATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286820.10112022324434587No Hit
CCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG284520.10030934355163966Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)