FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36217565
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT17903364.9432809743007295Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1802120.4975817672999276Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG1289960.3561697204105246Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTTGCAAAGCTGAAACT898910.2481972490420049Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTCCGACCATAAACGATG696920.19242596789706873Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACCGAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT662690.1829747527201235No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAACTAACCTGTCTCACG647760.1788524435588091Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAA588050.16236596800475128Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCCGAGCCGCCTGGATA541920.1496290543000337Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTTCCGGGAAACCAAAGT539770.14903541969207484Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGCCGTTCTTAGTTGGTG503150.13892430371837533Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACCGAGATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT466750.12887393175107162No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTAGGAATAATGGAATAG457920.1264358882216405Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACCGAGAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT456360.12600515799447037No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGACGGACCAGAGCGAAAG455190.12568211032409274Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGATTGACAGATTGATAGC437430.12077841235323247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG421080.11626402824154523Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGACGGACAGGATTGACAGATT401550.11087161712831882Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGCGAAAGCATTTGCCAA371940.1026960260856852Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACCGAGATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371050.10245028896890224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)