FastQCFastQC Report
Fri 24 Feb 2023
EGAF00002373024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30384891
Sequences flagged as poor quality0
Sequence length150
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT4195851.3809001322400662Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1145130.3768748092596416Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1138110.3745644504698075Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAGCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT964910.3175624358830183No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAACTAACCTGTCTCACG615700.2026336049716288Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAGCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT605030.19912199125545654No Hit
TATCAGCATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT523410.17225995643690148No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT453880.1493768728675051No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGCCGTTCTTAGTTGGTG429740.14143213480673666Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAGCATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420090.13825621424806164No Hit
TATCAGCATTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT399290.13141070672262736No Hit
TATCAGCAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379290.12482848794817135No Hit
TATCAGCATTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377480.12423279714908309No Hit
TATCAGCATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT375870.12370292853773937No Hit
TATCAGCATTATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355170.11689033210617737No Hit
TATCAGCATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT334470.11007773567461539No Hit
TATCAGCAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT332980.10958736037591842No Hit
TATCAGCATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323070.10632587097317546No Hit
TATCAGCATTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322210.10604283556587384No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTTGCAAAGCTGAAACT322130.10601650669077602Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAGCATAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT317480.10448614082571499No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)