FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31809933
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT6307041.982726590464683Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG3162020.9940354165474036Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1884940.5925633354839194Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1114640.3504062708965781Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCTCCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1013710.31867718803431616No Hit
ACCTCCAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT471950.14836560642865862No Hit
ACCTCCAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT401680.12627502233343277No Hit
ACCTCCAATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344720.10836866585038077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)