FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25070799
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT6956282.7746542900367874Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG2516971.0039448682907952Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATACCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT659840.2631906545938165No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC588710.23481900197915512Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG544410.2171490425973261Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATACCAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344300.13733108386374124No Hit
CATACCAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292320.11659779969517527No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGCGCCGCTAGAGGTGAA254500.10151252060215553Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC254490.10150853189800613Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATACCAATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251710.10039967214447373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)