FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26365965
Sequences flagged as poor quality0
Sequence length150
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1184500.44925342197791734No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG611800.23204157329344857Illumina Single End PCR Primer 1 (100% over 50bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA317130.12028006560730851No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA281000.10657679322566044No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)