FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33526555
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT5063731.5103639488160951Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5056341.5081597259247186Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG2727940.8136654660760702Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG2087970.6227809567669569Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAGCCATTGTGGCTCCGGC1793400.5349192602699562Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC1221280.364272440159748Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCATTGTGGCTCCGGCC835120.24909210027692974Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC644110.1921193513619279Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC641520.19134682940135067Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA633830.189053125201799No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT382600.11411849502580863Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC347690.10370585346451491Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)