FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27741201
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT8689533.1323553727900966Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG3492971.259127173333267Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1147600.4136807198794313Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGAGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT726930.2620398446339796No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC449370.16198649798903803Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAATTTGAAGTAGATAGA398010.1434725194485992Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGAGATCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339430.12235591386256132No Hit
AAGAGATCTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318620.11485443618681108No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC314230.11327195242916845Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTCCGACCATAAACGATG282480.10182688197241353Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTCCTCGTTCATGGGGAA280080.10096174278828086Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)