FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32304091
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT3834451.1869858836145553Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3349011.036713894843845Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG2955220.9148129257065305Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATCAAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1531960.4742309573112582No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC549140.16999085348044618Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA524760.16244382174381566Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATCAAGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT395250.12235292427822841No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTTACTGAGAACTCATA367490.11375958543455068Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATCAAGTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337830.10457808579105352No Hit
CATCAAGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330450.10229354542122854No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)