FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36185730
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT10611352.932468130392837Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG5637471.5579262875172064Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1327480.3668517948926276Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACAGCAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT766710.21188186613894483No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTTACTGAGAACTCATA696690.19253169688714308Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTACTGAGAACTCATAAG476540.1316927971330135Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC442910.12239907831070425Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAATTTGAAGTAGATAGA430280.118908752151746Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACAGCAGATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT413080.11415549720842995No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCACTTTGCTCGGGTCACG404390.11175399805392901Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACAGCAGAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380780.10522932658813294No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)