FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35819669
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT5416421.5121356928228455Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4927411.375615726655654Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG4028821.1247507619347348Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1544370.43115138780316475Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCTCCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1424230.3976111560383207No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1175670.32821911335919934Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1175240.3280990675821153Clontech Universal Primer Mix Long (96% over 26bp)
ACCTCTGCTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG855280.23877384238251892Clontech SMART CDS Primer II A (95% over 23bp)
ACCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT713060.19906939955251962Clontech Universal Primer Mix Long (96% over 26bp)
ACCTCCAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT657770.18363374602931143No Hit
ACCTCCAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT586220.1636586870749699No Hit
ACCTCCAATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT458540.12801346656776755No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT432870.12084701285207297Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTTACTGAGAACTCATA384650.10738513524510794Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCTCCAATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT369160.10306069550782282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)