FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31655319
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT6564772.073828414112649Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG3855411.2179343383018821Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGTTACTGAGAACTCATA1283570.40548319857399Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1107940.35000121148676466No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1017970.32157944767512847Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCACTTTGCTCGGGTCACG834590.26364921484443105Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT658290.20795557296389905No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTACTGAGAACTCATAAG655860.20718792945981684Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT563050.17786900204670186No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG486430.15366453896736912Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTCAATTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420450.13282128036681606No Hit
AGAGTCAATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417130.13177248348058032No Hit
AGAGTCAATTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381120.12039682809704114No Hit
AGAGTCAATTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377330.11919955695281416No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCAGTTACTGAGAACTCAT329760.10417206662804442Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)