FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37826468
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT5613841.4841036704775081Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAG3554090.9395775466004387Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1139890.3013471942450456Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCC1128500.29833607515245675Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACTCACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT407350.10768914507164665No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)