FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002373397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4832
Sequences flagged as poor quality0
Sequence length150
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG350.7243377483443708Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.6415562913907285No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.22764900662251655No Hit
TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.22764900662251655No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATCGGAAGAGCACACGTC90.18625827814569537Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.14486754966887416No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.12417218543046359No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.12417218543046359No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.12417218543046359No Hit
GTTTGGGTTTTAGAAGTTTGTTTTATAGTCGGTTGGTGGTAGAATTGGGT50.10347682119205298No Hit
CATGTGGTTTGGTATGATTTTGTTGGTGTTGGAGTGGTTGATTTTGTTGG50.10347682119205298No Hit
TATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.10347682119205298No Hit
TAAACAACCCATAAAAAAACTAAAATATATAACCTTTCTCAAATATACTT50.10347682119205298No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA50.10347682119205298Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)