FastQCFastQC Report
Wed 15 Mar 2023
EGAF00002373424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002373424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28311263
Sequences flagged as poor quality0
Sequence length150
%GC40

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2452840.8663831069634725Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA767340.2710370074270442Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA471730.16662273244397469Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGTATCATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402210.14206713420026512No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGAT388390.13718568472201328Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAATTTGAAGTAGATAGA371930.13137174417121555Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGTATCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365560.12912175624238312No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC354220.1251162832262199Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGATGTGGTGACGTCGTG342770.12107195641536726Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGTATCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333300.1177269979089241No Hit
ACGTATCAAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308320.10890365435127355No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATCGATGTGGTGACGTCGT305270.10782634458943073Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT301880.10662894128036605Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGTATCATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293490.10366545639450984No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTCGTGATCGATGTGGTGA293100.10352770203152012Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)